Maximal Lactate steady-state (MLSS) demarcates sustainable from unsustainable exercise and is used for evaluation/monitoring of exercise capacity. Still, its determination is physically challenging and time-consuming. This investigation aimed at validating a simple, submaximal approach based on blood lactate accumulation ([∆lactate]) at the third minute of cycling in a large cohort of men and women of different ages. 68 healthy adults (40♂, 28♀, 43 ± 17 years (range 19-78), VO_2max 45 ± 11 ml^-1·kg^-1·min^-1 (25-68)) performed 3-5 constant power output (PO) trials with a target duration of 30 minutes to determine the PO corresponding to MLSS. During each trial, [∆lactate] was calculated as the difference between the third minute and baseline. A multiple linear regression was computed to estimate MLSS based on [∆lactate], subjects` gender, age and the trial PO. The estimated MLSS was compared to the measured value by paired t-test, correlation, and Bland-Altman analysis. The group mean value of estimated MLSS was 180 ± 51 W, not significantly different from (p = 0.98) and highly correlated with (R² = 0.89) measured MLSS (180 ± 54 watts). The bias between values was 0.17 watts, and imprecision 18.2 watts. This simple, submaximal, time- and cost-efficient test accurately and precisely predicts MLSS across different samples of healthy individuals (adjusted R² = 0.88) and offers a practical and valid alternative to the traditional MLSS determination.

• Lactate accumulation from a submaximal test can be used to predict the maximal lactate steady state (MLSS).

• This approach was previously proposed in young men, and it is here extended to women and elderly people.

• Within the limitations of lactate measurements, this 3-min submaximal test offers an economical and practical approach to obtain a first estimate of the MLSS.